Contract Research

 

  • HERPEPS expression and purification system technology
  • Phase Ia
    • Construction of expression vector with SBP fused to target protein(TP)
    • Transformation & screening of expression strains
    • Flask level expression & purification of SBP-tagged protein
    • Performance report including :
  • Expression titer of SBP-tagged TP
  • Recovery rate analysis
  • SDS-PAGE analysis
  • Western blot analysis by antibodies for SBP or TP(provided by customer)
  • 1~3 mg protein will be provided to validate activity assay.

 

  • Phase II
    • Pilot scale expression in 30L fermentor
    • Performance report including :
  • Expression titer of SBP-tagged TP
  • Recovery rate analysis
  • SDS-PAGE analysis
  • Western blot analysis by antibodies for SBP or TP(provided by customer)
    • The fermentation broth can be provided for evaluation
    • Purified protein can be provided on request

 

  • Phase II
    • Scale up expression in 1500-8000L fermentor for the estimation of production cost
    • Performance report including :
  • Expression titer of SBP-tagged TP
  • Recovery rate analysis
  • SDS-PAGE analysis
  • Western blot analysis by antibodies for SBP or TP(provided by customer)
    • The fermentation broth can be provided for evaluation
    • Purified protein can be provided on request

 

  • Phase III
    • Turnkey technology transfer
    • Fermentation parameters
    • Downstream processing detail
    • Licensing of SBP technology